DNA
2ABH
Part:BBa_K4138000:Design
Designed by: Carlos Classen Group: iGEM22_Aachen (2022-09-28)
2ABH - Phosphate Binding Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 645
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We considered codon optimization for our desired E. coli strain when back-translating the amino acid sequence.
Source
We took the amino acid sequence from the Protein Data Base and back-translated it into a nucleotide sequence [1].
References
[1] https://www.rcsb.org/structure/2ABH (Source Last accessed 29.09.2022)