Part:BBa_K4134012:Design
AggC (N-terminal 1000bp)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed two short silver-binding peptides (Atox1 and AgBP2) on the C-terminus of the membrane protein, BpfA to "capture" silver ions. During the bio-oxidative electron transfer process, silver ions are reduced and aggregated into silver nanoparticles. The silver short peptides on the membrane protein enriched silver ions so that the silver nanoparticles (AgNPs) were more concentrated in the outer membrane and periplasmic space. Considering that silver is much more conductive than cytochromes, this approach may help speed up the process of electron transfer and improve cell efficiency.
Source
AggC is a gene downstream to the chromosomal BpfA on the genome of S. oneidensis. Reference: Sivakumar K, Mukherjee M, Cheng H I, et al. Surface display of roGFP for monitoring redox status of extracellular microenvironments in Shewanella oneidensis biofilms[J]. Biotechnology and Bioengineering, 2015, 112(3): 512-520.