Coding

Part:BBa_K4133012:Design

Designed by: Luis Alejandro Chaves Martinez   Group: iGEM22_Costa_Rica   (2022-10-04)

LysKB317 (Endolysin) for Lactobacillus casei


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 615
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was taken from the GenBank Accession KP027015.1 and codon optimized using the GenSmart™ Codon Optimization tool from GenScript, adding the RFC10 restriction sites as undesired inside the sequence.


Source

This part was de-novo synthesized


References