Part:BBa_K4133008

RBS_Lp (Lactobacillus plantarum WCFS1)

This RBS was obtained from the Lactobacillus plantarum WCFS1 genome (GenBank Accession: NC_004567.2), belonging to the ctsR-clpC operon

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

The repressor systems that Fiocco et al. (2010) found in L. plantarum is explained by the most studied clpC operon in Bacillus subtilis, which behave similarly, according to their studies, in these putative regions for each specie. The clpC operon is regulated by a class III stress induction mechanism in which the first gene ctsR, containing a predicted helix-turn-helix DNA-binding motif, acts as a negative regulator for a PclpC promoter. The deletion of the ctsR gene ended up in the high expression of the clpC operon under unstressed conditions, but once they tried the wildtype system under stress conditions like high temperatures (over 42˚C) a further increase in clpC operon was found, demonstrating that the ctsR protein acts as a global repressor and gets inactivated or dissociated under inducing stress conditions like 42˚C (Krüger et al., 1998).

References

Krüger, E., & Hecker, M. (1998). The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes. Journal of bacteriology, 180(24), 6681–6688. https://doi.org/10.1128/JB.180.24.6681-6688.1998

Fiocco, D., Capozzi, V., Collins, M., Gallone, A., Hols, P., Guzzo. J., Weidmann, S., Rieu. A., Msadek, T. & Spano, G. (2010). Characterization of the CtsR Stress Response Regulon in Lactobacillus plantarum. Journal of Bacteriology, 192(3): 896-900. https://doi.org/10.1128/JB.01122-09