Coding

Part:BBa_K4131007:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


pfa E gene (Moritella marina), codon optimized for E.coli expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 835


Design Notes

The sequence is codon optimized for E.Coli expression, and the restriction enzyme sites of XbaI, SpeI, EcoRI, PstI, and NotI are eliminated.


Source

The sequence is extracted from the region 1159170-1160033 in Moritella marina genome (WP_019440098.1)

References