Coding
Part:BBa_K4131002:Design
Designed by: Chun Ying Yu Group: iGEM22_CCU_Taiwan (2022-10-09)
AccE gene (C. glutamicum), codon optimized for E. Coli expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is codon optimized for E.Coli expression, and the restriction enzyme sites of XbaI, SpeI, EcoRI, PstI, and NotI are eliminated.
Source
The sequence is extracted and modified from the region 1507618-1507866 in Corynebacterium glutamicum genome (CP025534.1)