Part:BBa_K4129115

Minimal gpdA promoter fused with six LexO bindings sites (6xLexO-Pmin)

Minimal promoter and LexO

The promoter gpdA from Aspergillus nidulans was minimised to the minimum amount of nucleotides (180 bp) for transcription and was named Pmin. The transcription from Pmin was minor, but can still assemble the RNA polymerase if that process is initiated by a transcription factor. This system was used to make a tetracycline inducible system, TetOn, (Wanka et. al (2016)).

LexO is the name of the binding motif for LexA. LexA is the main regulator for the SOS response in E. coli (Radman (1975)). It has been shown in Aspergillus niger, that repeating LexO sites increase binding affinity and expression of a minimal promoter. The consensus number of LexO binding sites are six, 6xLexO (Rantasalo et. al (2018)).

All the fungal synthetic transcriptions (BBa_K4129100-BBa_K4129114) were characterised by this synthetic promoter.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 11
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 11
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 11
  
• 1000
  COMPATIBLE WITH RFC[1000]