Plasmid

Part:BBa_K4129019

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-11)


Integratable gene expression cassette with Lactaldehyde oxidoreductase

An integratable gene expression cassette with fucO with pyrG as selection in A. niger integration site 1


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6603
    Illegal XbaI site found at 5419
    Illegal XbaI site found at 6233
    Illegal XbaI site found at 6634
    Illegal XbaI site found at 10545
    Illegal XbaI site found at 13289
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7441
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6603
    Illegal NheI site found at 1026
    Illegal NheI site found at 2943
    Illegal NheI site found at 8097
    Illegal NheI site found at 9360
    Illegal NheI site found at 9368
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7441
    Illegal NotI site found at 8088
    Illegal NotI site found at 10502
    Illegal NotI site found at 13328
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6603
    Illegal BglII site found at 2177
    Illegal BglII site found at 8410
    Illegal BamHI site found at 5514
    Illegal BamHI site found at 6628
    Illegal BamHI site found at 7179
    Illegal BamHI site found at 10174
    Illegal XhoI site found at 2733
    Illegal XhoI site found at 3115
    Illegal XhoI site found at 5294
    Illegal XhoI site found at 7039
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6603
    Illegal XbaI site found at 5419
    Illegal XbaI site found at 6233
    Illegal XbaI site found at 6634
    Illegal XbaI site found at 10545
    Illegal XbaI site found at 13289
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7441
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6603
    Illegal XbaI site found at 5419
    Illegal XbaI site found at 6233
    Illegal XbaI site found at 6634
    Illegal XbaI site found at 10545
    Illegal XbaI site found at 13289
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7441
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 1267
    Illegal NgoMIV site found at 4772
    Illegal NgoMIV site found at 6150
    Illegal NgoMIV site found at 10056
    Illegal NgoMIV site found at 10181
    Illegal AgeI site found at 3322
    Illegal AgeI site found at 3470
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1468
    Illegal BsaI site found at 5120
    Illegal BsaI site found at 7232
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 3149
    Illegal BsaI.rc site found at 5864
    Illegal SapI site found at 2355
    Illegal SapI site found at 10334
    Illegal SapI.rc site found at 6370
    Illegal SapI.rc site found at 8181
    Illegal SapI.rc site found at 10421


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