Composite

Part:BBa_K4129015:Design

Designed by: Christian Wistrm   Group: iGEM22_DTU-Denmark   (2022-10-06)


Integratable gene expression cassette with Oxidoreductase, arz7774


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6934
    Illegal XbaI site found at 6564
    Illegal XbaI site found at 6965
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6934
    Illegal NheI site found at 1026
    Illegal NheI site found at 2943
    Illegal NheI site found at 5618
    Illegal NheI site found at 8428
    Illegal NheI site found at 9691
    Illegal NheI site found at 9699
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7772
    Illegal NotI site found at 8419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6934
    Illegal BglII site found at 2177
    Illegal BglII site found at 5638
    Illegal BglII site found at 8741
    Illegal BamHI site found at 5845
    Illegal BamHI site found at 6959
    Illegal BamHI site found at 7510
    Illegal BamHI site found at 10505
    Illegal XhoI site found at 2733
    Illegal XhoI site found at 3115
    Illegal XhoI site found at 7370
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6934
    Illegal XbaI site found at 6564
    Illegal XbaI site found at 6965
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6934
    Illegal XbaI site found at 6564
    Illegal XbaI site found at 6965
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal PstI site found at 2173
    Illegal PstI site found at 7772
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 1267
    Illegal NgoMIV site found at 6481
    Illegal NgoMIV site found at 10387
    Illegal NgoMIV site found at 10512
    Illegal AgeI site found at 3322
    Illegal AgeI site found at 3470
    Illegal AgeI site found at 5441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1468
    Illegal BsaI site found at 7563
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 3149
    Illegal BsaI.rc site found at 6195
    Illegal SapI site found at 2355
    Illegal SapI site found at 10665
    Illegal SapI.rc site found at 6701
    Illegal SapI.rc site found at 8512
    Illegal SapI.rc site found at 10752


Design Notes

Source

BBa_K4129013, BBa_K4129010, BBa_K678041, BBa_K4129014

References