Other

Part:BBa_K4129014

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-06)


A. niger downstream homology arm of integration site 1

This is a 2 kb homology arm, facilitating homologous recombination to insert a cassette using crispr cas9

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 912
    Illegal NheI site found at 920
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1726
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1608
    Illegal NgoMIV site found at 1733
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1886
    Illegal SapI.rc site found at 1973


[edit]
Categories
Parameters
None