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Part:BBa_K4129013

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-06)


A. niger upstream homology arm of integration site 1

This is a 2 kb homology arm, facilitating homologous recombination to insert a cassette using crispr cas9

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1026
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 637
    Illegal PstI site found at 900
    Illegal PstI site found at 1021
    Illegal NgoMIV site found at 625
    Illegal NgoMIV site found at 1267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1468
    Illegal BsaI.rc site found at 1540


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Categories
Parameters
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