Coding

Part:BBa_K4129012

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-06)


Gene expression cassette with Oxidoreductase, hmfH

Expression cassette with BBa_K4129009

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2712
    Illegal XbaI site found at 4792
    Illegal PstI site found at 141
    Illegal PstI site found at 2530
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal NheI site found at 3975
    Illegal PstI site found at 141
    Illegal PstI site found at 2530
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BglII site found at 3516
    Illegal BamHI site found at 2365
    Illegal BamHI site found at 2403
    Illegal BamHI site found at 4073
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3368
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2712
    Illegal XbaI site found at 4792
    Illegal PstI site found at 141
    Illegal PstI site found at 2530
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2712
    Illegal XbaI site found at 4792
    Illegal PstI site found at 141
    Illegal PstI site found at 2530
    Illegal NgoMIV site found at 3139
    Illegal NgoMIV site found at 4709
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2629
    Illegal BsaI site found at 3447
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 2507
    Illegal BsaI.rc site found at 4423
    Illegal SapI site found at 323


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Parameters
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