Coding

Part:BBa_K4129011

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-06)


Gene expression cassette with Lactaldehyde oxidoreductase, fucO

Expression cassette with BBa_K4129008

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3387
    Illegal XbaI site found at 4201
    Illegal PstI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
    Illegal PstI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 145
    Illegal BamHI site found at 3482
    Illegal XhoI site found at 701
    Illegal XhoI site found at 1083
    Illegal XhoI site found at 3262
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3387
    Illegal XbaI site found at 4201
    Illegal PstI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3387
    Illegal XbaI site found at 4201
    Illegal PstI site found at 141
    Illegal NgoMIV site found at 2740
    Illegal NgoMIV site found at 4118
    Illegal AgeI site found at 1290
    Illegal AgeI site found at 1438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3088
    Illegal BsaI.rc site found at 1117
    Illegal BsaI.rc site found at 3832
    Illegal SapI site found at 323


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Categories
Parameters
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