
Coding
Part:BBa_K4129010:Design
Designed by: Christian Wistrm Group: iGEM22_DTU-Denmark (2022-10-06)
arz_7774 expression cassette
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4532
Illegal PstI site found at 141 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 911
Illegal NheI site found at 3586
Illegal PstI site found at 141 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 145
Illegal BglII site found at 3606
Illegal BamHI site found at 3813
Illegal XhoI site found at 701
Illegal XhoI site found at 1083 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4532
Illegal PstI site found at 141 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4532
Illegal PstI site found at 141
Illegal NgoMIV site found at 4449
Illegal AgeI site found at 1290
Illegal AgeI site found at 1438
Illegal AgeI site found at 3409 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1117
Illegal BsaI.rc site found at 4163
Illegal SapI site found at 323
Design Notes
Source
BBa_K4129024, BBa_K4129007 and BBa_K678036.