Coding

Part:BBa_K4129009

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-02)


Oxidoreductase, oxidising furfural and furfuryl alcohol til furoic acid

This coding sequence produces a putative FAD-dependent oxidoreductase from Cupriavidus basilensis. This enzyme is hypothesised to oxidise both furfural and furfuryl alcohol to furoic acid, which is a part of a larger conversion pathway to channel furfural towards the citric acid cycle.

Paper: Koopman, F., Wierckx, N., Winde, J.h., & Ruijssenaars, H.J. Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14. Proceedings of the National Academy of Sciences. 107(11), 4919-4924 (2010)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 393
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1656
    Illegal PstI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1197
    Illegal BamHI site found at 46
    Illegal BamHI site found at 84
    Illegal XhoI site found at 1049
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 393
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 393
    Illegal PstI site found at 211
    Illegal NgoMIV site found at 820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 310
    Illegal BsaI site found at 1128
    Illegal BsaI.rc site found at 188


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Parameters
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