Coding

Part:BBa_K4129008

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-02)


Lactaldehyde oxidoreductase, reduction of furfural to furfuryl alcohol

This coding sequence produces a NADH-dependent oxidoreductase from Escherichia coli, and has been shown to reduce furfural to furfuryl alcohol.

Paper: Zheng, H., Wang, W., Yomano, L.P., Geddes, R.D.,,Shanmugam, K.T.& Ingram, L.O. Improving Escherichia coli FucO for Furfural Tolerance by Saturation Mutagenesis of Individual Amino Acid Positions. Applied and Environmental Microbiology, 79(10), 3202-3208, (2013)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1068
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 943
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1068
    Illegal NgoMIV site found at 421
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 769


[edit]
Categories
Parameters
None