Coding

Part:BBa_K4129007

Designed by: Christian WistrÝm   Group: iGEM22_DTU-Denmark   (2022-10-02)


Aldehyde dehydrogenase, oxidising furfural to furoic acid

This coding sequence produces a putative NAD-dependent aldehyde dehydrogenase named arz7774, identified in Amorphotheca resinae. It has been shown to be upregulated in Amorphotheca resinae while induced by furfuryl alcohol, a derivative of furfural and it is hypothesised to convert furfural to furoic acid, which is a part of a larger conversion pathway to channel furfural towards the citric acid cycle.

Paper: Wang, X., Gao, Q. & Bao, J. Transcriptional analysis of Amorphotheca resinae ZN1 on biological degradation of furfural and 5-hydroxymethylfurfural derived from lignocellulose pretreatment. Biotechnol Biofuels 8, 136 (2015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1273
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None