Regulatory

Part:BBa_K4129000

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-01)


Promoter of the Saccharomyces cerevisiae FDH1 gene


The part consists of the 1000 bp upstream of Formate Dehydrogenase 1 (FDH1) CDS from Saccharomyces cerevisiae. FDH1 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified the part from gDNA through PCR and used it to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 278
    Illegal XhoI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193
    Illegal BsaI.rc site found at 345


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