Part:BBa_K4124003

ACC-1 (Acetyl Coenzyme A Carboxylase 1)

Contribution

Acetyl-CoA carboxylase 1 (ACC1) is an important regulator of endogenous fatty acid synthesis and promotes the conversion of acetyl CoA to malonic acid monoacyl CoA; This Coenzyme A derivative prevents fatty acids from entering the mitochondria and promotes fatty acid chain elongation, controlling the proportion of T cells differentiated to TH17 and Treg, so it has a role in the pathogenesis of autoimmune diseases.

Because the fragrant flavor of Baijiu is produced by FAEEs, the concentration of this substance is also the standard for the quality of Baijiu, thus the output of FAEEs from S. cerevisiae is important; However, due to the single strain fermentation process of Baijiu, the production of FAEEs is not enough causing the flavor becoming insufficient. Therefore, if the FAEEs produced by yeast are increased, the flavor of Baijiu can be more abundant and fragrant.

The content of this project is to improve the FAEEs production ability of S.cerevisiae cells by constructing FAS1, FAS2 and ACC1 plasmids.

Figure 1. An overview of the experimental principle..

In this experiment, we will construct the plasmids ACC1. Under the function of Malonyl-CoA, ACC1, FAS1, and FAS2 synthesize fatty acids, fatty Acyl-CoAs produce FAEEs; Therefore, as long as we successfully construct one of the three plasmids, the production of FAEEs can be significantly increased.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 306
  Illegal BglII site found at 544
  Illegal BglII site found at 3728
  Illegal BglII site found at 5439
  Illegal BglII site found at 6292
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4027
  Illegal AgeI site found at 5266
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 3632
  Illegal SapI.rc site found at 3317