Coding

Part:BBa_K4121028:Design

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


phytoene synthase encoding gene CrtB from Pantoea agglomerans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 598
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 643
    Illegal BglII site found at 859
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 598
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.


Source

National Center for Biotechnology Information (NCBI)

References