Coding

Part:BBa_K412004:Design

Designed by: Lagnajeet Pradhan   Group: iGEM10_UTDallas   (2010-10-27)


Codes for transcription factor AlkS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 888
    Illegal BsaI.rc site found at 359
    Illegal BsaI.rc site found at 661


Design Notes

Plan to execute site-directed mutagenesis to remove restriction sites on the gene to make it compatible with iGEM.


Source

alkS gene from Pseudomonas putida OCT (GenBank: http://www.ncbi.nlm.nih.gov/nuccore/10945174?from=4355&to=7003&report=gbwithparts) Original publication: http://www.ncbi.nlm.nih.gov/pubmed/11535032

  • Provided in glycerol stock by Dr. van der Meer as was used in the following publication: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC168716/pdf/634053.pdf

References