Part:BBa_K412001:Experience

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Applications of BBa_K412001

• All images were taken with Olympus IX81 automated inverted microscope specially equipped for live cell imaging. All microscope images were taken from live DH5α E.coli cells. The filter sets we used are: 545/30x (excitation) and 620/60m (emission) filters for DsRed, 470/40x (excitation) and 525/50m (emission) for GFP. Data collection and processing was performed by the SlideBook software.
• The data was taken using cells that were grown overnight in 2mL of LB broth that had 50ug/mL of the appropriate antibiotic at 37C and 220rpm. This was used in a 1:50 dilution. Each sample had 40uL from the broth grown overnight and 2mL of the LB broth with the appropriate antibiotic. These were allowed to grow until a predetermined OD. Then the contaminants were added and the cells were allowed to grow for another 2 hours, before the images were taken.
• The images and the graph are from PyeaR+RFP incubated with potassium nitrite. The graph is of the average of all the maximum intensities of the cells in the picture vs the concentration of nitrite added to the sample. We also took data from potassium nitrate and aluminum nitrate nonahydrate, but the cells were not responsive to these.

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