Composite

Part:BBa_K412001:Design

Designed by: Kristina Ehrhardt   Group: iGEM10_UTDallas   (2010-10-15)


PyeaR+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
    Illegal AgeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We knew we wanted a nitrate sensitive promoter followed by a reporter to work in E.coli. Therefore we digested PyeaR with SpeI and PstI and digested RFP with XbaI and PstI and then ligated the two parts together to form this composite part.


Source

We got both subparts from the registry, when we looked for a nitrate detector and a reporter.

References