Composite

Part:BBa_K4119026

Designed by: Chengtai Yin   Group: iGEM22_NJTech_China   (2022-09-30)


Pvgb-separate FNR binding site from -35->Bs2->Cpa fdx terminator

PvgbF3/PvgbF7-Bs2-Cpa fdx terminator


Document

Results:

After modification, both experimental groups had lower expression levels than the positive control group, and we found that the pMTL82151-PvgbF3-Bs2 experimental group did not achieve the purpose of the transformation. However，the pMTL82151-PvgbF7-Bs2 experimental group showed aerobic inhibition and microoxide expression characteristics.

Figure. Expression effect of PvgbF3-bs2, PvgbF7-bs2 and Pvgb at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 357
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None