Composite

Part:BBa_K4119018

Designed by: Chengtai Yin   Group: iGEM22_NJTech_China   (2022-09-30)


PthlA-FNRbindingsite-7->Bs2->Cpa fdx terminator

PthlF7（BBa_K4119016）-Bs2-Cpa fdx terminator


Document

Results:

The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.

Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 841
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 841
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 841
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 841
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None