Regulatory
Part:BBa_K4119015
Designed by: Chengtai Yin Group: iGEM22_NJTech_China (2022-09-30)
PthlA-FNRbindingsite-3
PthlAF3 was modified based on the PthlA constitutive promoter from Clostridium acetobutyricum.A FNR binding site was inserted at the distance of 3 bp from the - 35 region of the PthlA promoter.
Results:
The new promoter thlAF3 has a satisfatory performence. It is inhibited in aerobic condition while strengthed in microaerobic condition.
Fig.2. Expression effect of PthlA and PthlAF3-bs2 at different oxygen concentrations
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |