Part:BBa_K4117088
CYP2C19
“T7 promoter+ pmrA+ RBS+ pmrB+ rrnB T1 terminator+ pmrC promoter” is the Δ9-THC sensing pathway. To alert vaccinators that they have ingested Δ9-THC, we design a Δ9-THC sensing system. In this pathway, anti-THC can specifically bind to Δ9-THC, and then promote PmrB phosphorylation of PmrA. PmrC promoters activate phosphorylated PmrA proteins and initiate transcription to express pigment proteins. Pigment proteins can be excreted in the stool, acting as a warning.Outer membrane protease T (ompT) is a 33.5 kDa endoprotease located on the outer membrane of Escherichia coli. OmpT signal peptide is an excretion tag linked on the N-terminus of protein. It can transport proteins across the outer membrane with the help of OmpT.Δ9-THC is hydroxylated to 11-OH-Δ9-THC via CYP2C9 (BBa_K4117002) and CYP2C19(BBa_K4117022), remaining psychoactive.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4467
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214
Illegal PstI site found at 3118
Illegal PstI site found at 4348 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4467
Illegal SpeI site found at 713
Illegal PstI site found at 1214
Illegal PstI site found at 3118
Illegal PstI site found at 4348
Illegal NotI site found at 3275 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4467
Illegal BglII site found at 4386
Illegal BamHI site found at 2887
Illegal BamHI site found at 2994 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4467
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214
Illegal PstI site found at 3118
Illegal PstI site found at 4348 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4467
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214
Illegal PstI site found at 3118
Illegal PstI site found at 4348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 732
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