Composite

Part:BBa_K4115040:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)


J23101-B0034-LuxI-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The function of LuxI is verified in E. coli, which will then be used to build communications and regulations between A.caulinodans ORS571 and S.elongatus HL7942.


Source

The sequence of LuxI is gained from the iGEM parts (BBa_C0161). The LuxI fragment was synthesized by the company. The plasmid was constructed by our team.

References