Regulatory

Part:BBa_K4115014

Designed by: Shenxi Cao   Group: iGEM22_ShanghaiTech_China   (2022-09-21)


PnifH（with RBS）

The promoter of the nifH coding sequence on the nitrogen-fixing gene cluster is regulated by the nifA protein.


Usage and Biology

The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP (BBa_K4115000) was expressed downstream to verify whether the artificially designed system could regulate nif gene cluster as expected in A. caul.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 272


[1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2

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