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DNA
Part:BBa_K4115008:Design
Designed by: Shenxi Cao Group: iGEM22_ShanghaiTech_China (2022-09-21)
NifA_downstream
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 138
Illegal BamHI site found at 477 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is used as a one-sided homology arm on the designed plasmid.
Source
Sequences were obtained from the Azorhizobium caulinodans ORS571 genome.
References
Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2