Plasmid

Part:BBa_K4099006:Design

Designed by: Nixue Song   Group: iGEM21_Shanghai_HS_ID   (2021-10-12)


pNCas9-LSEI-2094


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13411
    Illegal XbaI site found at 14374
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8143
    Illegal NheI site found at 13795
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6903
    Illegal BglII site found at 7540
    Illegal BglII site found at 13141
    Illegal BglII site found at 13195
    Illegal BglII site found at 14371
    Illegal XhoI site found at 3061
    Illegal XhoI site found at 7536
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13411
    Illegal XbaI site found at 14374
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13411
    Illegal XbaI site found at 14374
    Illegal AgeI site found at 6696
    Illegal AgeI site found at 14076
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

No

References