Composite

Part:BBa_K4088899

Designed by: Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-21)


dCas13a-Nlact-int

N-terminal fragment of β-lactamase fused with dCas13a and N-terminal intein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 622
    Illegal BglII site found at 924
    Illegal BglII site found at 1689
    Illegal BglII site found at 1956
    Illegal BglII site found at 2166
    Illegal BglII site found at 4020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 622
    Illegal PstI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None