Composite
Part:BBa_K4088899
Designed by: Pavel Ivanov-Rostovtsev Group: iGEM21_Moscow_City (2021-10-21)
dCas13a-Nlact-int
N-terminal fragment of β-lactamase fused with dCas13a and N-terminal intein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 622
Illegal BglII site found at 924
Illegal BglII site found at 1689
Illegal BglII site found at 1956
Illegal BglII site found at 2166
Illegal BglII site found at 4020 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |