Coding

Part:BBa_K4088897

Designed by: Ruslan Vasiliev, Yaroslav Ugolkov, Alice Potapova, Pavel Ivanov-Rostovtsev   Group: iGEM21_Moscow_City   (2021-10-21)


C-lact

C-terminal beta-lactamase - half of the gene that encodes beta-lactamase (AmpR). It can fuse with N-terminal beta-lactamase to fully assemble beta-lactamase that has the ability to hydrolyze nitrocefine allows beta-lactamase to be used as a reporter protein where solution color is changing.
For more information, see our part BBa_K4088890.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 138


[edit]
Categories
Parameters
None