Regulatory

Part:BBa_K4085018

Designed by: yuanze Liu   Group: iGEM21_Ulink-SIP   (2021-10-21)


rcsB

Colanic acid synthesis in E. coli K-12 is regulated by the rcs system, which includes three positive regulators (RcsA, RcsB, and RcsF) and two negative regulators (RcsC and Lon). RcsB and RcsC act as the effector and the sensor, respectively, of a two-component regulatory system by stimulating capsule synthesis from colanic acid synthesis cps genes (36). The environmental signals which activate RcsC have yet to be determined. RcsA is an auxiliary factor which may interact with RcsB to form a heterodimer required for increased transcription of cps genes. In our project, the gene is one of the pair of heterologous positive regulators, RcsA and RcsB. They can attempte to promote GDP-L-fucose formation and thus 2′-FL production.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 367
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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