Plasmid_Backbone
azl

Part:BBa_K4074046

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)


pSB_AZL

Contents: azl operon
What is it?: A basic part - plasmid backbone
What does it do?: It codes for azl homology arms, which allow for integration into the B. subtilis genome at the azl operon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1826
    Illegal XbaI site found at 1799
    Illegal PstI site found at 1787
    Illegal PstI site found at 2972
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1826
    Illegal PstI site found at 1787
    Illegal PstI site found at 2972
    Illegal NotI site found at 2965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1826
    Illegal BamHI site found at 1805
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1826
    Illegal XbaI site found at 1799
    Illegal PstI site found at 1787
    Illegal PstI site found at 2972
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1826
    Illegal XbaI site found at 1799
    Illegal PstI site found at 1787
    Illegal PstI site found at 2972
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None