Plasmid_Backbone
amyE gibso

Part:BBa_K4074043:Design

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)


pSB_AMY_GIBSON


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1187
    Illegal suffix found at 3886
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal XbaI site found at 2450
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal SpeI site found at 3887
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
    Illegal PstI site found at 3901
    Illegal NotI site found at 1193
    Illegal NotI site found at 3875
    Illegal NotI site found at 3894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal BamHI site found at 2456
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1187
    Illegal suffix found at 3887
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal XbaI site found at 2450
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1187
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal XbaI site found at 1202
    Illegal XbaI site found at 2450
    Illegal SpeI site found at 3887
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
    Illegal PstI site found at 3901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

-


Source

pSB1C from iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab

References