Plasmid_Backbone
amyE

Part:BBa_K4074042

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-05)


pSB_AMY

Contents: amyE
What is it?: A basic part - backbone
What does it do?: It codes for amyE homology arms, which will allow for integration into the B. subtilis genome at the amyE locus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1692
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 1000
    Illegal XbaI site found at 2370
    Illegal PstI site found at 1880
    Illegal PstI site found at 1906
    Illegal PstI site found at 2358
    Illegal PstI site found at 3802
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1692
    Illegal EcoRI site found at 2397
    Illegal PstI site found at 1880
    Illegal PstI site found at 1906
    Illegal PstI site found at 2358
    Illegal PstI site found at 3802
    Illegal NotI site found at 991
    Illegal NotI site found at 3795
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1692
    Illegal EcoRI site found at 2397
    Illegal BamHI site found at 2376
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1118
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1692
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 1000
    Illegal XbaI site found at 2370
    Illegal PstI site found at 1880
    Illegal PstI site found at 1906
    Illegal PstI site found at 2358
    Illegal PstI site found at 3802
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1692
    Illegal EcoRI site found at 2397
    Illegal XbaI site found at 1000
    Illegal XbaI site found at 2370
    Illegal PstI site found at 1880
    Illegal PstI site found at 1906
    Illegal PstI site found at 2358
    Illegal PstI site found at 3802
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
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