Composite

Part:BBa_K4072004:Design

Designed by: Barney Geddes   Group: iGEM21_NDSU   (2021-10-19)


Constitutive chiA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2035
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2076
    Illegal AgeI site found at 1398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 148
    Illegal BsaI.rc site found at 2186


Design Notes

Adapted chiA to MoClo golden gate sequence. DVK_AE backbone did not show correct size by restriction digest but worked for the golden gate cloning.


Source

CIDAR MoClo and chiA from Bacillus cereus

References