Composite

Part:BBa_K4072003:Design

Designed by: Barney Geddes   Group: iGEM21_NDSU   (2021-10-19)


Constitutive chiA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Adapted chiA to MoClo golden gate sequence. DVK_AE backbone did not show correct size by restriction digest but worked for the golden gate cloning.


Source

CIDAR MoClo and chiA from Bacillus cereus

References