Composite
Part:BBa_K4072003:Design
Designed by: Barney Geddes Group: iGEM21_NDSU (2021-10-19)
Constitutive chiA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Adapted chiA to MoClo golden gate sequence. DVK_AE backbone did not show correct size by restriction digest but worked for the golden gate cloning.
Source
CIDAR MoClo and chiA from Bacillus cereus