Composite

Part:BBa_K4063137

Designed by: Brooke Dillingham   Group: iGEM21_Gaston_Day_School   (2021-10-20)


T4 early promoter driving GFP production

It is a group 1 T4 early promoter, located at position 128.6 on the T4 chromosome. Promoter was originally identified by Liebig and Ruger (1989), and further characterized by Wilkens and Ruger (1995).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 80
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 80
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 80
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 740


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