Composite

Part:BBa_K4062015:Design

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-15)


lacY ORF and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 787
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the genome, lacY is directly downstream of lacZ (3.1kb). The extra bases ensure proper spacing between genes.


Source

Sequences from Escherichia coli K12 from biocyc.org

References