Part:BBa_K4061110

SH3 domain with linker

This part can be put in 3' end of the desired protein CDS. A protein engineered with this domain should be used together with another protein modified with leucine zipper domain (BBa_K406111). Upon the expression of a scaffold protein (BBa_K4061015), the interaction between these two proteins can be enhanced. Our team used these domains on the EnvZ and OmpR proteins to enhance their interaction and prevent cross-talk from other TCS species present in E. coli. This protein contains an inert peptide chain of 12 amino acid Glycine-Serine that is meant to separate the additional domain from the parent protein and provide flexibility to the domain for interacting with the scaffold. This part was inspired from Whitaker, W. R. (2012) "Engineering robust control of two-component system phosphotransfer using modular scaffolds".

Teams are recommended to use SWISS-MODEL or any protein modelling software to confirm whether this part interferes with the parent protein sequence. Since scaffold protein has an SH3 ligand with a high affinity (Kd 0.1uM), the scaffold may still competitively bind to the domain.

This part is yet to be characterised, wet lab work is in progress

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 151