Reporter
Part:BBa_K4061090:Design
Designed by: Diya Agrawal Group: iGEM21_HKUST (2021-10-15)
eGFP antisense characterisation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1325
Design Notes
Since we ordered the construct through Twist Bioscience, we needed to reduce the complexity of the DNA sequence. By nature if are to synthesise this construct, DNA will have secondary structure since we are joining a sense and an antisense strand in the same fragment. We needed to reduce the complexity on other ends, therefore we used two different terminators and optimised the sequence till it gave low complexity scores.
Source
Biobricks