Coding

Part:BBa_K4060506:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


QPAS1 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use PCR technique to clone the gene fragment from template pKA-207I10(Plasmid#79845) from Addgene. We add restriction enzyme cutting site that according to igem part assembly protocol RFC#10 to both sides of the gene fragment, then using these restriction enzyme cutting site to insert the gene fragment into vector pSB1C3.


Source

This part is cloned from Addgene plasmid pKA-207I10.

References