Part:BBa_K4060103:Experience

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Applications of BBa_K4060103

We made a series of liquid cultures with different concentrations of L-arabinose. Then, we cultured E. coli  DH5-alpha with pBad and pBad-J23106 separately. After making the bacteria shake at 24°C for 24 hrs, we tested the RFP intensity and OD600 of these liquid cultures. According to the figure, we can see that pBad-J23106 significantly outshines the original BioBrick_BBa_K206000. When the L-arabinose is lower than 0.025 M, both the pBad and pBad-J23106 promoters were induced. The RFP intensity of the improved part pBad-J23106 reached about 17000 at the L-arabinose concentration of 0.025, which is eight times higher than the original part pBad. The expression rate of pBad-J23106 still exceeds the origin part pBad at the concentration of L-arabinose higher than 0.025. We implemented this design in our kil-switch. Please visit our project result page for more information.

User Reviews

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