Coding
MazF

Part:BBa_K4060001

Designed by: Yi Rong Chen   Group: iGEM21_NYCU-Taipei   (2021-09-30)


MazF gene

mazF gene from E.coli MG1655

MazF is a toxin gene which will cleave ACA RNA sequence and lead to the cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal SpeI site found at 363
    Illegal NotI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None