Composite

Part:BBa_K4059029:Design

Designed by: Ann-Sofie Nielsen   Group: iGEM21_SDU-Denmark   (2021-10-21)


CPR from Phanerochaete chrysosporium with N-terminal substitution (15), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Taken from a water-soluble cytochrome b5 reductase. The sequence is taken on the basis of the specified topology

Source

https://www.uniprot.org/uniprot/A0A5F9CGD1

References