RNA
ICE2 multi

Part:BBa_K4058017:Design

Designed by: Sarah Cumberland   Group: iGEM21_Guelph   (2021-10-20)


ICE2 multiplex gRNA (ICE2 sgRNAs A, B, C, D, E)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gRNA sequences were designed using the guide RNA design software CHOPCHOP. The parameters were set to find optimal CRISPR/Cas9 target sites for CRISPR activation. Target sequences were selected based on the predicted binding efficiency provided by CHOPCHOP.


Source

This sequence comes from the promoter sequence of the ICE2 gene found in Arabidopsis thaliana.


References

A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517