RNA
CLE18 mult

Part:BBa_K4058016:Design

Designed by: Sarah Cumberland   Group: iGEM21_Guelph   (2021-10-20)


CLE18 multiplex gRNA (CLE18 sgRNAs A, B, C, D, E)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9
    Illegal BsaI.rc site found at 2897


Design Notes

The gRNA sequences were designed using the guide RNA design software CHOPCHOP. The parameters were set to find optimal CRISPR/Cas9 target sites for CRISPR activation. Target sequences were selected based on the predicted binding efficiency provided by CHOPCHOP.


Source

These sequences come from the promoter sequence of the CLE18 gene found in Arabidopsis thaliana.


References

A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517