Generator

Part:BBa_K4054107

Designed by: Xinyan Han   Group: iGEM21_XJTLU-CHINA   (2021-10-19)


pT7_T7 RBS_gp24

It is used to process the efficient expressiong of pg24 in vivo to help the recombination of phage. It is recommended to use BL21(DR3) or use T7 polymerase-based cell-free system to perform the experssion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 563
    Illegal PstI site found at 703
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1352
    Illegal PstI site found at 563
    Illegal PstI site found at 703
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 563
    Illegal PstI site found at 703
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 563
    Illegal PstI site found at 703
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1102
    Illegal SapI site found at 439


[edit]
Categories
Parameters
None